Weakly Supervised Set-Consistency Learning Improves Morphological Profiling of Single-Cell Images

• URL: http://arxiv.org/abs/2406.05308v1
• Date: Sat, 8 Jun 2024 00:53:30 GMT
• Title: Weakly Supervised Set-Consistency Learning Improves Morphological Profiling of Single-Cell Images
• Authors: Heming Yao, Phil Hanslovsky, Jan-Christian Huetter, Burkhard Hoeckendorf, David Richmond,
• Abstract summary: We propose a set-level consistency learning algorithm, Set-DINO, to improve learned representations of perturbation effects in single-cell images. We conduct experiments on a large-scale Optical Pooled Screening dataset with more than 5000 genetic perturbations.
• Score: 0.6491172192043603
• License: http://arxiv.org/licenses/nonexclusive-distrib/1.0/
• Abstract: Optical Pooled Screening (OPS) is a powerful tool combining high-content microscopy with genetic engineering to investigate gene function in disease. The characterization of high-content images remains an active area of research and is currently undergoing rapid innovation through the application of self-supervised learning and vision transformers. In this study, we propose a set-level consistency learning algorithm, Set-DINO, that combines self-supervised learning with weak supervision to improve learned representations of perturbation effects in single-cell images. Our method leverages the replicate structure of OPS experiments (i.e., cells undergoing the same genetic perturbation, both within and across batches) as a form of weak supervision. We conduct extensive experiments on a large-scale OPS dataset with more than 5000 genetic perturbations, and demonstrate that Set-DINO helps mitigate the impact of confounders and encodes more biologically meaningful information. In particular, Set-DINO recalls known biological relationships with higher accuracy compared to commonly used methods for morphological profiling, suggesting that it can generate more reliable insights from drug target discovery campaigns leveraging OPS.

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