Differentiable model-based adaptive optics for two-photon microscopy

• URL: http://arxiv.org/abs/2104.14308v1
• Date: Thu, 29 Apr 2021 12:50:08 GMT
• Title: Differentiable model-based adaptive optics for two-photon microscopy
• Authors: Ivan Vishniakou, Johannes D. Seelig
• Abstract summary: Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. We extend this approach to two-photon scanning microscopy.
• Score: 0.0
• License: http://arxiv.org/licenses/nonexclusive-distrib/1.0/
• Abstract: Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be combined with the optimization techniques of machine learning frameworks to find aberration corrections, as was demonstrated for focusing a laser beam through aberrations onto a camera [arXiv:2007.13400]. Here, we extend this approach to two-photon scanning microscopy. The developed sensorless technique finds corrections for aberrations in scattering samples and will be useful for a range of imaging application, for example in brain tissue.

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