Tensorial tomographic differential phase-contrast microscopy
- URL: http://arxiv.org/abs/2204.11397v1
- Date: Mon, 25 Apr 2022 02:11:29 GMT
- Title: Tensorial tomographic differential phase-contrast microscopy
- Authors: Shiqi Xu, Xiang Dai, Xi Yang, Kevin C. Zhou, Kanghyun Kim, Vinayak
Pathak, Carolyn Glass, Roarke Horstmeyer
- Abstract summary: T2DPC extends differential phase-contrast microscopy, a quantitative phase imaging technique, to highlight the vectorial nature of light.
We demonstrate accurate volumetric reconstructions of refractive index, birefringence, and orientation for various validation samples, and show that the reconstructed polarization structures of a biological specimen are predictive of pathology.
- Score: 14.650158000199932
- License: http://creativecommons.org/licenses/by/4.0/
- Abstract: We report Tensorial Tomographic Differential Phase-Contrast microscopy
(T2DPC), a quantitative label-free tomographic imaging method for simultaneous
measurement of phase and anisotropy. T2DPC extends differential phase-contrast
microscopy, a quantitative phase imaging technique, to highlight the vectorial
nature of light. The method solves for permittivity tensor of anisotropic
samples from intensity measurements acquired with a standard microscope
equipped with an LED matrix, a circular polarizer, and a polarization-sensitive
camera. We demonstrate accurate volumetric reconstructions of refractive index,
birefringence, and orientation for various validation samples, and show that
the reconstructed polarization structures of a biological specimen are
predictive of pathology.
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