Z-upscaling: Optical Flow Guided Frame Interpolation for Isotropic Reconstruction of 3D EM Volumes

• URL: http://arxiv.org/abs/2410.07043v1
• Date: Wed, 9 Oct 2024 16:34:39 GMT
• Title: Z-upscaling: Optical Flow Guided Frame Interpolation for Isotropic Reconstruction of 3D EM Volumes
• Authors: Fisseha A. Ferede, Ali Khalighifar, Jaison John, Krishnan Venkataraman, Khaled Khairy,
• Abstract summary: We propose a novel optical flow based approach to enhance the axial resolution of anisotropic 3D EM volumes to achieve isotropic 3D reconstruction. Pixel level motion is estimated between neighboring 2D slices along z, using spatial gradient flow estimates to interpolate and generate new 2D slices resulting in isotropic voxels.
• Score: 0.0
• License: http://arxiv.org/licenses/nonexclusive-distrib/1.0/
• Abstract: We propose a novel optical flow based approach to enhance the axial resolution of anisotropic 3D EM volumes to achieve isotropic 3D reconstruction. Assuming spatial continuity of 3D biological structures in well aligned EM volumes, we reasoned that optical flow estimation techniques, often applied for temporal resolution enhancement in videos, can be utilized. Pixel level motion is estimated between neighboring 2D slices along z, using spatial gradient flow estimates to interpolate and generate new 2D slices resulting in isotropic voxels. We leverage recent state-of-the-art learning methods for video frame interpolation and transfer learning techniques, and demonstrate the success of our approach on publicly available ultrastructure EM volumes.

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