Related papers: A Compact Incubation Platform for Long-Term Cultivation of Biological Samples for Nitrogen-Vacancy Center Widefield Microscopy

A Compact Incubation Platform for Long-Term Cultivation of Biological Samples for Nitrogen-Vacancy Center Widefield Microscopy

URL: http://arxiv.org/abs/2512.14482v1
Date: Tue, 16 Dec 2025 15:14:04 GMT
Title: A Compact Incubation Platform for Long-Term Cultivation of Biological Samples for Nitrogen-Vacancy Center Widefield Microscopy
Abstract summary: Nitrogen-vacancy (NV) centers in diamond provide a versatile quantum sensing platform for biological imaging through magnetic field detection.<n>Here, we present a purpose-built compact incubation platform that maintains precise environmental control of temperature, CO$$ atmosphere, and humidity.<n>We demonstrate sustained viability and proliferation of HT29 colorectal cancer cells over 90 hours of continuous incubation, with successful magnetic field imaging of immunomagnetically labeled cells.
Score: 0.0
License: http://creativecommons.org/licenses/by/4.0/
Abstract: Nitrogen-vacancy (NV) centers in diamond provide a versatile quantum sensing platform for biological imaging through magnetic field detection, offering unlimited photostability and the ability to perform long-term observations without photobleaching or phototoxicity. However, conventional stage-top incubators are incompatible with the unique requirements for NV widefield magnetometry to study cellular dynamics. Here, we present a purpose-built compact incubation platform that maintains precise environmental control of temperature, CO$_2$ atmosphere, and humidity while accommodating the complex constraints of NV widefield microscopy. The system employs a 3D-printed biocompatible chamber with integrated heating elements, temperature control, and humidified gas flow to create a stable physiological environment directly on the diamond sensing surface. We demonstrate sustained viability and proliferation of HT29 colorectal cancer cells over 90 hours of continuous incubation, with successful magnetic field imaging of immunomagnetically labeled cells after extended cultivation periods. This incubation platform enables long-term cultivation and real-time monitoring of biological samples on NV widefield magnetometry platforms, opening new possibilities for studying dynamic cellular processes using quantum sensing technologies.

Related papers

Toward a CMOS-integrated quantum diamond biosensor based on NV centers [27.327120599253046]
We report progress toward a CMOS-integrated quantum diamond biosensing platform.<n>The system integrates on-chip active quenching and digital readout with external FPGA-based photon counting.<n>Magnetic field sensitivity of approximately 90 nT/$sqrtmathrmHz$ per pixel is estimated.
arXiv Detail & Related papers (2026-02-24T00:37:17Z)
Quantum magnetic imaging of current density in lithium-ion batteries [0.0]
We present a sensitive quantum-magnetometry method that uses optically pumped magnetometers (OPMs) to perform real-time imaging of internal dynamics in open-circuit configuration.<n>The measurement results are benchmarked against superconducting-quantum-interference-device (SQUID) magnetometry and validated with three-dimensional finite element simulations.<n>This work establishes OPM-based magnetic imaging of battery current density as a powerful diagnostic tool with potential impact on cell development, manufacturing quality assurance, and second-life assessment.
arXiv Detail & Related papers (2025-11-30T22:32:34Z)
Modeling Microenvironment Trajectories on Spatial Transcriptomics with NicheFlow [80.00833033784079]
Understanding of cellular microenvironment is essential for deciphering tissue development and disease data.<n>NicheFlow is a flow-based generative model that infers the temporal trajectory of cellular microenvironments across spatial slides.
arXiv Detail & Related papers (2025-11-02T15:41:38Z)
COMS-Integrated Atomic Vapor Cells with Ultra-long Optical Access for Highly Sensitive and Scalable Quantum Sensors [6.798957182622441]
We describe a micromachining paradigm for wafer-level atomic vapor cells functionalized by CMOS-compatible non-magnetic heaters and temperature sensors.<n>The integrated vapor cells achieved an ultra-long optical access of 5 mm, nearly four time that of previously microfabricated vapor cells.<n>Our achievements broaden the potential applications of microfabricated atomic vapor cells and pave the way for scalable manufacturing of ultrasensitive, chip-scale quantum sensors.
arXiv Detail & Related papers (2025-07-08T13:53:51Z)
A Low-Temperature Tunable Microcavity featuring High Passive Stability and Microwave Integration [0.0]
This work presents a closed-cycle cryogenic fiber-based microcavity setup, which is in particular designed for a low passive vibration level.<n>At temperatures below 10 Kelvin, a stability level of around 25 picometer is reproducibly achieved in different setup configurations.
arXiv Detail & Related papers (2024-09-03T12:53:41Z)
Q-BiC: A biocompatible integrated chip for in vitro and in vivo spin-based quantum sensing [0.23906118847859378]
Optically addressable spin-based quantum sensors enable nanoscale measurements of temperature, magnetic field, pH, and other physical properties of a system. We present the Quantum Biosensing Chip (Q-BiC), which facilitates microfluidic-compatible microwave delivery and includes on-chip temperature control.
arXiv Detail & Related papers (2024-06-03T10:26:15Z)
Microwave-based quantum control and coherence protection of tin-vacancy spin qubits in a strain-tuned diamond membrane heterostructure [54.501132156894435]
Tin-vacancy center (SnV) in diamond is a promising spin-photon interface with desirable optical and spin properties at 1.7 K. We introduce a new platform that overcomes these challenges - SnV centers in uniformly strained thin diamond membranes. The presence of crystal strain suppresses temperature dependent dephasing processes, leading to a considerable improvement of the coherence time up to 223 $mu$s at 4 K.
arXiv Detail & Related papers (2023-07-21T21:40:21Z)
High speed microcircuit and synthetic biosignal widefield imaging using nitrogen vacancies in diamond [44.62475518267084]
We show how to image signals from a microscopic lithographically patterned circuit at the micrometer scale. Using a new type of lock-in amplifier camera, we demonstrate sub-millisecond spatially resolved recovery of AC and pulsed electrical current signals. Finally, we demonstrate as a proof of principle the recovery of synthetic signals replicating the exact form of signals in a biological neural network.
arXiv Detail & Related papers (2021-07-29T16:27:39Z)
Laser threshold magnetometry using green light absorption by diamond nitrogen vacancies in an external cavity laser [52.77024349608834]
Nitrogen vacancy (NV) centers in diamond have attracted considerable recent interest for use in quantum sensing. We show theoretical sensitivity to magnetic field on the pT/sqrt(Hz) level is possible using a diamond with an optimal density of NV centers.
arXiv Detail & Related papers (2021-01-22T18:58:05Z)
Towards an Automatic Analysis of CHO-K1 Suspension Growth in Microfluidic Single-cell Cultivation [63.94623495501023]
We propose a novel Machine Learning architecture, which allows us to infuse a neural deep network with human-powered abstraction on the level of data. Specifically, we train a generative model simultaneously on natural and synthetic data, so that it learns a shared representation, from which a target variable, such as the cell count, can be reliably estimated.
arXiv Detail & Related papers (2020-10-20T08:36:51Z)
Optimisation of a diamond nitrogen vacancy centre magnetometer for sensing of biological signals [44.62475518267084]
We present advances in biomagnetometry using nitrogen vacancy centres in diamond. We show magnetic field sensitivity of approximately 100 pT/$sqrtHz$ in the DC/low frequency range using a setup designed for biological measurements.
arXiv Detail & Related papers (2020-04-05T18:44:34Z)

This list is automatically generated from the titles and abstracts of the papers in this site.