Cell segmentation from telecentric bright-field transmitted light
microscopic images using a Residual Attention U-Net: a case study on HeLa
line
- URL: http://arxiv.org/abs/2203.12290v1
- Date: Wed, 23 Mar 2022 09:20:30 GMT
- Title: Cell segmentation from telecentric bright-field transmitted light
microscopic images using a Residual Attention U-Net: a case study on HeLa
line
- Authors: Ali Ghaznavi, Renata Rychtarikova, Mohammadmehdi Saberioon, Dalibor
Stys
- Abstract summary: Living cell segmentation from bright-field light microscopic images is challenging due to the image complexity and temporal changes in the living cells.
Recently developed deep learning (DL)-based methods became popular in medical and microscopic image segmentation tasks due to their success and promising outcomes.
The main objective of this paper is to develop a deep learning, UNet-based method to segment the living cells of the HeLa line in bright-field transmitted light microscopy.
- Score: 0.0
- License: http://arxiv.org/licenses/nonexclusive-distrib/1.0/
- Abstract: Living cell segmentation from bright-field light microscopic images is
challenging due to the image complexity and temporal changes in the living
cells. Recently developed deep learning (DL)-based methods became popular in
medical and microscopic image segmentation tasks due to their success and
promising outcomes. The main objective of this paper is to develop a deep
learning, UNet-based method to segment the living cells of the HeLa line in
bright-field transmitted light microscopy. To find the most suitable
architecture for our datasets, we have proposed a residual attention U-Net and
compared it with an attention and a simple U-Net architecture. The attention
mechanism highlights the remarkable features and suppresses activations in the
irrelevant image regions. The residual mechanism overcomes with vanishing
gradient problem. The Mean-IoU score for our datasets reaches 0.9505, 0.9524,
and 0.9530 for the simple, attention, and residual attention U-Net,
respectively. We achieved the most accurate semantic segmentation results in
the Mean-IoU and Dice metrics by applying the residual and attention mechanisms
together. The watershed method applied to this best - Residual Attention -
semantic segmentation result gave the segmentation with the specific
information for each cell.
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